Description
Hayem’s solution is used in routine manual counting of erythrocytes. It is important to correctly prepare and dilute the sample of blood in the specified volume during every counting method. Basic advantages of Hayem’s solution (compared to other solutions for erythrocyte counting) are isotonicity (no hemolysis occurs), fixation (erythrocytes do not lose their shape, no autolysis occurs, so counting may be conducted a few hours after blood dilution). Hayem’s solution also prevents agglutination and has a longer shelf life.
Product description
- HAYEM’S SOLUTION – isotonic solution for manual counting of erythrocytes. Testing sample
- Uncoagulated venous or capillary blood
- Other necessary preparations:
Neubauer or Bürker-Türk’s hemocytometer
RBC pipette
Cover glass
- Preparation
RBC pipette filling
Draw blood into the RBC pipette to the 0.5 mark, then draw Hayem’s solution to the 101 mark. Dilution is 1/200.
Carefully stir the blood sample and Hayem’s solution. Use the preparation within a few hours.
Filling the hemocytometer
Discard the first two drops and then fill the hemocytometer.
Counting under the microscope
Counting is carried out under the microscope with a 10x magnifying factor lens. It is necessary to lower the condenser and move the front lens
outwards.
Count the erythrocytes in the center part of the hemocytometer. Four diagonal fields are most commonly counted (64 squares). For more precision,
count one peripheral field (total of 80 squares).
Result
Calculation of red blood cells
One side of a square is 1/20 mm in length; depth is 1/10 mm (after positioning the cover glass). Calculate the mean value of erythrocytes per square
and then number of erythrocytes in 1 mm3
of blood. Do not omit the dilution factor; multiply the result with 200.
The results are expressed as a mean value of double counting.
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